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Merck
모든 사진(1)

주요 문서

AB5818

Sigma-Aldrich

Anti-Potassium Channel Kir4.1 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
클론:
polyclonal
application:
WB
종 반응성:
rat, mouse, human
기술:
western blot: suitable
citations:
8

생물학적 소스

rabbit

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat, mouse, human

제조업체/상표

Chemicon®

기술

western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... KCNJ10(3766)

특이성

Recognizes Kir4.1. The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Other species have not been tested.

면역원

CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Synthetic peptide corresponding to amino acids 356-375 of rat, mouse or human Kir4.1 (Accession P49655).

애플리케이션

This Anti-Potassium Channel Kir4.1 Antibody is validated for use in WB for the detection of Potassium Channel Kir4.1.
Western blot: 1:200-1:600 using ECL on rat brain membranes or human platelets.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

물리적 형태

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for

분석 메모

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

Zuzanna Setkowicz et al.
Journal of anatomy, 231(3), 366-379 (2017-06-10)
Astrocytes participate in neuronal development and excitability, and produce factors enhancing or suppressing inflammatory processes occurring due to neurodegenerative diseases, such as epilepsy. Seizures, in turn, trigger the release of inflammatory mediators, causing structural and functional changes in the brain.
Meritxell Espino Guarch et al.
eLife, 7 (2018-01-23)
Age-related hearing loss (ARHL) is the most common sensory deficit in the elderly. The disease has a multifactorial etiology with both environmental and genetic factors involved being largely unknown. SLC7A8/SLC3A2 heterodimer is a neutral amino acid exchanger. Here, we demonstrated
Yue Yang et al.
PloS one, 6(1), e16547-e16547 (2011-02-10)
The endothelial-blood/tissue barrier is critical for maintaining tissue homeostasis. The ear harbors a unique endothelial-blood/tissue barrier which we term "blood-labyrinth-barrier". This barrier is critical for maintaining inner ear homeostasis. Disruption of the blood-labyrinth-barrier is closely associated with a number of
Steven A Hill et al.
eLife, 8 (2019-06-14)
Astrocytes have emerged as integral partners with neurons in regulating synapse formation and function, but the mechanisms that mediate these interactions are not well understood. Here, we show that Sonic hedgehog (Shh) signaling in mature astrocytes is required for establishing

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