추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, rat
제조업체/상표
Chemicon®
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... SFTPC(6440) , SP5(389058)
일반 설명
Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.
특이성
Expected to cross-react with human.
Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.
면역원
GST-tagged recombinant protein corresponding to the N-terminal region of human Prosurfactant Protein C (proSP-C).
애플리케이션
- Western Blotting Analysis: A 1:500 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue lysate and GST-fusion Prosurfactant Protein C fragment.
- ELISA Analysis: A 1:50,000 dilution from a representative lot detected GST-fusion Prosurfactant Protein C fragment.
- Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
This rabbit polyclonal Anti-Prosurfactant Protein C (proSP-C), Cat. No. AB3786 is tested for use in Western Blotting, Immunohistochemistry (Paraffin) and ELISA, for the detection of Prosurfactant Protein C (proSP-C).
품질
Evaluated by Western Blotting in rat lung tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Prosurfactant Protein C (proSP-C) in rat lung tissue lysate.
표적 설명
20 kDa observed. 21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
결합
Replaces: 07-647
물리적 형태
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.
분석 메모
Control
Mouse lung epithelial cells.
Mouse lung epithelial cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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