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Merck
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Key Documents

AB3430

Sigma-Aldrich

Anti-Prosurfactant Protein B Antibody, reacts with both CT and NT

serum, Chemicon®

동의어(들):

proSP-B

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human, mouse

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... SFTPB(6439)

특이성

Reacts strongly with human and mouse proSP-B (detects both the N-terminal and C-terminal propeptides). Does not react with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways.

면역원

Epitope: reacts with both C-terminus and N-terminus
Full length recombinant peptide (381 a.a.) of the human SP-B (pre)proprotein.

애플리케이션

Detect Prosurfactant Protein B using this Anti-Prosurfactant Protein B Antibody, reacts with both C-terminus & N-terminus validated for use in ELISA, IH & WB.
Immunohistochemistry: 1:4,000-1:8,000 on human tissue with and without antigen retrieval (see protocol on back) and 1:1,000-1:2,000 on adult and fetal mouse lung (see notes on back).

Immunoblot: 1:1,000-1:5,000; proprotein 42-46kDa.

ELISA: 1:500-1:1,000

Optimal dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels

물리적 형태

Rabbit serum. Liquid. Contains no preservative.

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Daniela M Tomazela et al.
Analytical chemistry, 82(6), 2561-2567 (2010-02-25)
We describe a method to measure protein synthesis and catabolism in humans without prior purification and use the method to measure the turnover of surfactant protein-B (SP-B). SP-B, a lung-specific, hydrophobic protein essential for fetal-neonatal respiratory transition, is present in
Increased epithelial cell proliferation in very premature baboons with chronic lung disease.
William M Maniscalco, Richard H Watkins,
American Journal of Physiology. Lung Cellular and Molecular Physiology null
Jonathan M Charest et al.
Biomaterials, 52, 79-87 (2015-03-31)
The primary treatment for end-stage lung disease is lung transplantation. However, donor organ shortage remains a major barrier for many patients. In recent years, techniques for maintaining lungs ex vivo for evaluation and short-term (<12 h) resuscitation have come into
Shashi P Singh et al.
Environmental health perspectives, 121(8), 957-964 (2013-06-13)
Cigarette smoke (CS) exposure during gestation may increase the risk of bronchopulmonary dysplasia (BPD)-a developmental lung condition primarily seen in neonates that is characterized by hypoalveolarization, decreased angiogenesis, and diminished surfactant protein production and may increase the risk of chronic
Raffaele Gerlini et al.
Cell death & disease, 10(10), 747-747 (2019-10-05)
The serine-threonine kinase homeodomain-interacting protein kinase 2 (HIPK2) modulates important cellular functions during development, acting as a signal integrator of a wide variety of stress signals, and as a regulator of transcription factors and cofactors. We have previously demonstrated that

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