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Merck
모든 사진(1)

Key Documents

AB3242

Sigma-Aldrich

Anti-PGC-1 Antibody

Chemicon®, from rabbit

동의어(들):

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

mouse, rat, human

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

PGC-1 is a transcription factor that determines whether preadipocytes will differentiate into brown or white fat cells. It interacts with PPARgamma to promote the expression of a cassette of genes which define the brown adipocyte phenotype.

특이성

Immunogen peptide blocking prior to Western blotting completely abolished target band detection by this rabbit polyclonal antibody.

면역원

Epitope: C-terminus
Synthetic peptide from amino acids 777-797 (C-terminus) of mouse PGC-1.

애플리케이션

Anti-PGC-1 Antibody is a rabbit olyclponal antibody for the detection of PGC-1 also known as PPARgamma Coactivator 1-alpha & has been validated in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
ELISA Analysis: A 1:75,000 dilution of a previous lot was used in ELISA.

Immunocytochemistry Analysis: A 1:300 dilution of a previous lot was used in Immunocytochemistry.

Immunofluorescence Analysis: Representative lots detected PGC-1α in frozen mouse and rat muscle tissue sections (Gouspillou, G., et al. (2013). Longev. Healthspan. 2(1):13; Matsuura, T., et al. (2007). J. Orthop. Res. 25(11):1534-1540).

Immunohistochemistry Analysis: A representative lot detected PGC-1α in free-floating mouse forebrain sections (Tallaksen-Greene, S.J., et al. (2014). J Neurosci. 34(47):15658-15668).

Immunoprecipitation Analysis: Representative lots immunoprecipitated PGC-1α from mouse liver, skeletal muscle, and brown adipose tissue lysates (Boutant, M., et al. (2016). Cell Rep. 14(9):2068-2075; Park, R.D., et al. (2014). J. Exerc. Nutrition Biochem. 18(1):1-7; Kim, J.S., et al. (2014). J. Exerc. Nutrition Biochem. 18(3):259-266; Philip, A., et al. (2011). J. Biol. Chem. 286(35):30561-30570).

Western Blotting Analysis: Representative lots detected similar PGC-1α in human, mouse, and rat skeletal muscle tissue, as well as in nuclear and mitochondrial preparations from rat brown adipose tissue (Suwa, M., et al. (2015). J. Sports Sci. Med. 14(3):548-555; Lee, I., et al. (2015). Front. Pharmacol. 6:43; Vincent, G., et al. (2015). Front Physiol. 6:51; Lombardi, A., et al. (2015). PLoS One. 10(2):e0116498; Gouspillou, G., et al. (2014). FASEB J. 28(4):1621-1633; Saleem, A., et al. (2014). Am. J. Physiol. Cell Physiol. 306(3):C241-249).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

품질

Evaluated by Western Blotting in mouse BAT extracts.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant mouse PGC-1, as well as endogenous PGC-1 in 15 µg of nuclear extract and whole cell lysate from mouse brown adipose tissue (BAT). Immunogen peptide blocking completely blocked target band detection.

표적 설명

~100 kDa observed. 91.03/89.64/91.71/77.14 kDa (human isoform 1/B4/B5/L-PGG-1alpha), 90.59 kDa (mouse isoform 1; PGC-1a1), 90.62 kDa (rat) calculated.

물리적 형태

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 20 mM PBS and 0.1% sodium azide.

저장 및 안정성

Stable for 1 year at -70ºC from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles.

분석 메모

Control
BAT nuclear prep

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Long-term high-fat-diet feeding induces skeletal muscle mitochondrial biogenesis in rats in a sex-dependent and muscle-type specific manner.
Gomez-Perez, Y; Capllonch-Amer, G; Gianotti, M; Llado, I; Proenza, AM
Nutrition & Metabolism null
Metformin increases the PGC-1alpha protein and oxidative enzyme activities possibly via AMPK phosphorylation in skeletal muscle in vivo.
Suwa, M; Egashira, T; Nakano, H; Sasaki, H; Kumagai, S
Journal of Applied Physiology (1985)
Differential gene expression of muscle-specific ubiquitin ligase MAFbx Atrogin-1 and MuRF1 in response to immobilization-induced atrophy of slow-twitch and fast-twitch muscles.
Okamoto T, Torii S, Machida S
The Journal of Physiological Sciences : Jps null
Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle.
Masataka Suwa,Hiroshi Nakano,Zsolt Radak,Shuzo Kumagai
Metabolism, Clinical and Experimental null
Divergent skeletal muscle respiratory capacities in rats artificially selected for high and low running ability: a role for Nor1?
Stephenson, EJ; Stepto, NK; Koch, LG; Britton, SL; Hawley, JA
Journal of Applied Physiology (1985)

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