AB16502

Anti-AIF Antibody, NT

Chemicon^®, from rabbit

• 동의어(들): Apoptosis Inducing Factor
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: western blot: suitable
• NCBI 수납 번호: NM_004208.2; NM_145812.1; NM_145813.1
• UniProt 수납 번호: O95831
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... AIFM1(9131)

설명

특이성

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and is widely expressed (Susin et al. 1999).

면역원

Epitope: N-terminus

Rabbit anti-AIF (N-terminus of mature AIF) polyclonal antibody was raised against a peptide corresponding to amino acids 109 to 122 of human AIF (Susin et al. 1999).

애플리케이션

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-AIF Antibody, N-terminus is validated for use in WB for the detection of AIF.

Western blot: 0.25 to 1 μg/mL.

K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.

Optimal working dilutions must be determined by end user.

결합

Replaces: 04-430

물리적 형태

Format: Purified

Purified IgG by ion exchange chromatography. Liquid in PBS containing 0.02% sodium azide.

저장 및 안정성

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable