콘텐츠로 건너뛰기
Merck
모든 사진(3)

Key Documents

AB15282

Sigma-Aldrich

Anti-Cone Arrestin Antibody

Chemicon®, from rabbit

동의어(들):

Anti-Arrestin-C

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

분자량

antibody estimated mol wt ~46 kDa

정제법

affinity chromatography

종 반응성

rat, mouse

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Arr3(170735)
rat ... Arr3(171107)

일반 설명

Arrestin-C (UniProt: Q9EQP6; also known as Cone arrestin, cArr, Retinal cone arrestin-3) is encoded by the Arr3 gene (Gene ID: 170735) in murine species. Arrestins are a superfamily of multi-functional proteins that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C, a disulfide-linked, homodimeric protein, is predominantly found in inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves as a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Mutations in ARR3 gene in humans have been linked to X-lined myopia 26 that is characterized by typical tigroid fundus changes commonly seen in early onset high myopia. (Ref.: Gurevich, VV., et al. (2018). Protein Cell. 9; 986-1003; Xiao, X., et al. (2016). Mol. Vis. 22; 1257-1266).

특이성

This rabbit polyclonal antibody detects Arrestin-C. It targets an epitope within 12 amino acids from the C-terminal region.

면역원

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of mouse Cone Arrestin.

애플리케이션

Anti-Cone Arrestin , Cat. No. AB15282, is a rabbit polyclonal antibody that detects Arrestin-C and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Cone Arrestin in mouse cerebellum, rat retina, and mouse retina tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

품질

Evaluated by Western Blotting in Mouse Retina tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Arrestin-C in Mouse Retina tissue lysate.

표적 설명

Target molecular weight ~50 kDa observed. 41.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Phospholipid flippase ATP8A2 is required for normal visual and auditory function and photoreceptor and spiral ganglion cell survival.
Coleman, JA; Zhu, X; Djajadi, HR; Molday, LL; Smith, RS; Libby, RT; John, SW; Molday, RS
Journal of Cell Science null
Assessment of tropism and effectiveness of new primate-derived hybrid recombinant AAV serotypes in the mouse and primate retina.
Charbel Issa, P; De Silva, SR; Lipinski, DM; Singh, MS; Mouravlev, A; You, Q; Barnard et al.
Testing null
Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact visually-guided behavior.
Sarria, I; Pahlberg, J; Cao, Y; Kolesnikov, AV; Kefalov, VJ; Sampath, AP; Martemyanov, KA
eLife null
Development and plasticity of outer retinal circuitry following genetic removal of horizontal cells.
Keeley, PW; Luna, G; Fariss, RN; Skyles, KA; Madsen, NR; Raven, MA; Poche, RA; Swindell et al.
The Journal of Neuroscience null
Katharina Kranz et al.
PloS one, 8(2), e57163-e57163 (2013-03-08)
Retinitis pigmentosa (RP) relates to a group of hereditary neurodegenerative diseases of the retina. On the cellular level, RP results in the primary death of rod photoreceptors, caused by rod-specific mutations, followed by a secondary degeneration of genetically normal cones.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.