추천 제품
생물학적 소스
sheep
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... BDNF(627)
특이성
Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.
The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
면역원
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
애플리케이션
Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, WB, IH.
Immunohistochemistry: 1:200-1:2,000 (see suggested protocol).
Immunoblotting: 1:200-1:2,000 we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochemistry 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH 7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C.
ELISA: 1:200-1:2,000
Inhibition of biological activity in vitro: 1:10-1:50
Use neat for in vivo animal studies.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:200-1:2,000 we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochemistry 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH 7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C.
ELISA: 1:200-1:2,000
Inhibition of biological activity in vitro: 1:10-1:50
Use neat for in vivo animal studies.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
물리적 형태
Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any insoluble material.
저장 및 안정성
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Expression of brain-derived neurotrophic factor protein in the adult rat central nervous system
Yan, Q, et al
Neuroscience, 78, 431-448 (1997)
MeCP2 controls BDNF expression and cocaine intake through homeostatic interactions with microRNA-212.
Im, HI; Hollander, JA; Bali, P; Kenny, PJ
Nature Neuroscience null
Christopher R Hansebout et al.
Neural regeneration research, 7(28), 2165-2175 (2012-10-05)
Previous studies have shown that transplanted enteric glia enhance axonal regeneration, reduce tissue damage, and promote functional recovery following spinal cord injury. However, the mechanisms by which enteric glia mediate these beneficial effects are unknown. Neurotrophic factors can promote neuronal
Kosuke Fujita et al.
Molecular therapy. Methods & clinical development, 5, 130-141 (2017-05-10)
Retinal ganglion cell degeneration triggered by axonal injury is believed to underlie many ocular diseases, including glaucoma and optic neuritis. In these diseases, retinal ganglion cells are affected unevenly, both spatially and temporally, such that healthy and unhealthy cells coexist
Nicotinic alpha 7 receptor clusters on hippocampal GABAergic neurons: regulation by synaptic activity and neurotrophins.
Kawai, Hideki, et al.
The Journal of Neuroscience, 22, 7903-7912 (2002)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.