추천 제품
생물학적 소스
sheep
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, rat, chicken
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... BDNF(627)
특이성
Recognizes Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4. The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
면역원
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
애플리케이션
Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.
Immunohistochemistry: 1-5 μg/mL (see suggested protocol).
Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
ELISA: 1-5 μg/mL
Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Use neat for in vivo studies.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
ELISA: 1-5 μg/mL
Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Use neat for in vivo studies.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
표적 설명
~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
물리적 형태
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water.
저장 및 안정성
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability. Avoid repeated freeze/thaw cycles.
분석 메모
Control
Brain tissue
Brain tissue
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
ONO-5046 attenuation of delayed motor neuron death and effect on the induction of brain-derived neurotrophic factor, phosphorylated extracellular signal-regulated kinase, and caspase3 after spinal cord ischemia in rabbits.
Takashi Yamauchi, Yoshiki Sawa, Masahiro Sakurai, Takano Hiroshi, Goro Matsumiya et al.
The Journal of Thoracic and Cardiovascular Surgery null
Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes).
P G Vissio, M M Canepa, M C Maggese, P G Vissio, M M Canepa, M C Maggese
Tissue & cell null
Trk activation of the ERK1/2 kinase pathway stimulates intermediate chain phosphorylation and recruits cytoplasmic dynein to signaling endosomes for retrograde axonal transport.
Mitchell, DJ; Blasier, KR; Jeffery, ED; Ross, MW; Pullikuth, AK; Suo, D; Park, J; Smiley et al.
The Journal of Neuroscience null
Brain-derived neurotrophic factor augments peristalsis by augmenting 5-HT and calcitonin gene-related peptide release.
Grider John R JR, Piland Barbara E BE, Gulick Melisa A MA, Qiao Li Ya LY
Gastroenterology null
Upregulation of CREB-mediated transcription enhances both short- and long-term memory.
Suzuki, A; Fukushima, H; Mukawa, T; Toyoda, H; Wu, LJ; Zhao, MG; Xu, H; Shang, Y; Endoh et al.
The Journal of Neuroscience null
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