AB10554

Anti-Tbr1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): T-box, brain, 1, T-brain-1, T-box brain protein 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): canine (based on 100% sequence homology), canine, bovine, opossum, horse, rat, pig
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_033348
• UniProt 수납 번호: Q64336
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TBR1(10716)

설명

일반 설명

T-box brain protein 1 (UniProt: Q64336; also known as T-brain-1, TBR-1, TES-56) is encoded by the Tbr1 gene (Gene ID: 21375) in murine species. Tbr1 is a homodimeric nuclear protein that serves as a transcriptional repressor and is involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZ Family Zinc Finger 2 (FEZF2), it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons. Its T-box DNA binding domain is localized in amino acids 213-393. Its expression is observed in developing and adult cerebral cortex and in olfactory bulbs. It is detected around day 10 of embryonic development in the preplate and at day 12.5 in the cortical plate. It is highly expressed in glutamatergic early-born cortical neurons. Tbr1 null mice exhibit severe defects of frontal cortex and layer 6 differentiation. (Ref.: Han, W., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(7); 3041-3046; Bedogni, F., et al (2010). Proc. Natl Acad. Sci. USA. 107(29); 13129-13134; Hevner, RF., et al. (2001). Neuron. 29(2); 353-366).

특이성

This rabbit polyclonal antibody detects T-box brain protein 1 (Tbr1).

면역원

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of mouse T-box brain protein 1 (Tbr1).

KLH-conjugated linear peptide corresponding to mouse Tbr1 near the N-terminus.

애플리케이션

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Mouse brain tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution of this antibody detected Tbr1 in Mouse cerebral cortex and cerebellum tissue sections.


Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Tbr1 in Mouse fetal brain tissue lysate.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

품질

Evaluated by Immunohistochemistry in mouse frontal cortex tissue.

Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Tbr1 in mouse frontal cortex tissue.

표적 설명

~76 kDa observed; 73.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

분석 메모

Control
Mouse frontal cortex tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable