71077

X-Gal Solution - Novagen

Convenience for blue/white screening

• 동의어(들): X-Gal solution
• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• Quality Level: 100
• 양식: solution
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze
• 기술: tissue culture: suitable
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

애플리케이션

X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the Apc^Δ242 mutation in the tissue sample.

경고

Toxicity: Irritant (B)

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 188.6 °F
• Flash Point (°C): 87 °C