70527

100 mM IPTG Solution - Novagen

Convenient, ready-to-use solution

• 동의어(들): 100 mM IPTG Solution - Novagen, IPTG Solution, 100 mM, Isopropyl -β-D-1-thiogalactopyranoside, Isopropylthio -β-D-galactoside
• CAS Number: 367-93-1
• MDL number: MFCD00063273
• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• Quality Level: 100
• 형태: solution
• 분자량: 238.30 g/mol
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −20°C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

설명

애플리케이션

100 mM IPTG Solution - Novagen has been used to:

• induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
• induce expression in calcium-independent sortase from BL21(DE3) cells
• turn on bacterial protein production of the protein A/G-MNase

생화학적/생리학적 작용

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.

경고

Toxicity: Carcinogenic / Teratogenic (D)

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable