69182

DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

• 동의어(들): RNase-free DNase I
• UNSPSC 코드: 12352202
• NACRES: NA.85

속성

• 형태: liquid
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: DNA extraction: suitable
• 적합성: suitable for nucleic acid purification
• 응용 분야: diagnostic assay manufacturing
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable