567704

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

• 동의어(들): SCDase
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: bacterial (Pseudomonas spp.)
• Quality Level: 100
• 양식: liquid
• 특이 활성도: ≥1 units/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 외래 활성: α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

애플리케이션

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

물리적 형태

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

재구성

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable