553506-M

RAP, Human, Recombinant, E. coli

• 동의어(들): Receptor Associated Protein
• UNSPSC 코드: 12352202

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 100
• 양식: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 저장 온도: −70°C

설명

일반 설명

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.

경고

Toxicity: Standard Handling (A)

물리적 형태

150 mM NaCl, 100 mM Tris-HCl, pH 7.4.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Li, Y., et al. 2002. Biochemistry41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable