추천 제품
형태
lyophilized
특이 활성도
≥70,000 proteolytic units/g dry wt
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
solubility
water: 10 mg/mL
외래 활성
Nuclease, none detected
배송 상태
ambient
저장 온도
2-8°C
일반 설명
PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.
애플리케이션
Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
- in single-cell preparations for cardiac side population (CSP) analysis
- in aflatoxin plasma biomarker assay
- to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion
생화학적/생리학적 작용
Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.
경고
Toxicity: Harmful (C)
단위 정의
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
재구성
Unstable in solution; reconstitute just prior to use.
기타 정보
Note: 1 KU = 1000 units.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype
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