48-630MAG

MILLIPLEX^® NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay

• UNSPSC 코드: 12161503
• NACRES: NA.47

속성

• 종 반응성: human
• Quality Level: 200
• 제조업체/상표: Milliplex^®
• 기술: multiplexing: suitable
• 검출 방법: fluorometric (Luminex^® xMAP^®)
• 저장 온도: 2-8°C

설명

일반 설명

NF-κB (nuclear factor kappa B) is a transcription factor that plays a regulatory role in several physiological and pathological processes. The NF-κB pathway can be activated by numerous stimuli, including inflammatory cytokines, virus, bacteria, UV radiation, genotoxic stress, DNA damage, oxidative stress, etc. The IkBα/NF-κB complex is tightly regulated by both phosphorylation and ubiquitination. Phosphorylation and ubiquitination of IκBα leads to its degradation and subsequent release of phosphorylated NF-κB to the nucleus. Greater than 100 target genes, including cytokines, cyclins, p21, Bcl-2, and c-Myc are known to be transcribed by NF-kB.

특이성

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

애플리케이션

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.This is an overnight (4°C) incubation assay.This kit must be run using the provided Assay Buffer.This assay requires 25 μL diluted filtered cell lysate per well.The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:c-Myc;FADD (Ser194);IκBα (Ser32);IKKα/β (Ser177/Ser181);NFκB (Ser536);TNFR1

포장

96-well plate

저장 및 안정성

Recommended storage for kit components is 2 - 8°C.

법적 정보

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H410
• 예방조치 성명서: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids