444258

MMP-14 Substrate I, Fluorogenic

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

• 동의어(들): MMP-14 Substrate I, Fluorogenic, MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
• 실험식(Hill 표기법): C₆₆H₈₂N₁₆O₁₇S
• Molecular Weight: 1403.52
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥97% (HPLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: yellow
• solubility: DMSO: 5 mg/mL
• 형광: λ_ex ~328 nm; λ_em ~400 nm
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (k_cat/k_m = 730,000 M^-1s^-1 for MMP-14 (MT1-MMP) and 36,000 M^-1s^-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (k_cat/K_m = 7.3 x 10⁵ M^-1s^-1 for MMP-14 (MT1-MMP) and 3.6 x 10⁴ M^-1s^-1 for MMP-11 at 25°C, pH 7.5).

생화학적/생리학적 작용

Cell permeable: no

Primary Target
MMP-14

Product does not compete with ATP.

Reversible: no

kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11

포장

Packaged under inert gas

경고

Toxicity: Standard Handling (A)

서열

MCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

기타 정보

Mucha, A., et al. 1998. J. Biol. Chem.273, 2763.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable