444248

MMP-13, Proenzyme, His•Tag^®, Human, Recombinant, S. frugiperda

• 동의어(들): Collagenase-3
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥50 mU/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).

Note: 1 mU = 1 milliunit.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

물리적 형태

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Herget, J., et al. 2003. Am. J. Physiol.285, 4199.
Nagase, H. 1997. Biol. Chem.378, 151.
Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Knauper, V., et al. 1996. J. Biol. Chem.271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.
Knight, C.G., et al. 1992. FEBS Lett.296, 263.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable