444213

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

• 동의어(들): Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
• 효소 위원회 번호: 3.4.24.24 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥600 mU/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• solubility: aqueous buffer: soluble
• 외래 활성: other MMP activity, none detected
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.

Note: 1 mU = 1 milliunit.

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

물리적 형태

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

제조 메모

Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

법적 정보

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable