407294

αA-Interferon, Human, Recombinant, E. coli

• 동의어(들): IFNA2A, IFNA2B, IFNA2C, rhIFN-αA
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 설명: Merck USA index - 14, 4990
• Quality Level: 100
• 분석: ≥95% (SDS-PAGE)
• 양식: liquid
• 특이 활성도: ≥2.8 x 10⁸ units/mg protein; ≥5 x 10⁶ units/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: cell based assay: suitable
• NCBI 수납 번호: NM_000605.4
• UniProt 수납 번호: P01563
• 배송 상태: wet ice
• 저장 온도: −70°C
• 유전자 정보: human ... IFNA1(3340)

설명

일반 설명

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.

애플리케이션

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.

생화학적/생리학적 작용

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

물리적 형태

In PBS containing 0.1% BSA.

제조 메모

Dilute only in buffers containing protein such as 0.1% BSA.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable