400050

Hygromycin B, Streptomyces sp., Cell Culture-Tested

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.

• 동의어(들): Hygromycin B, Streptomyces sp., Cell Culture-Tested
• 실험식(Hill 표기법): C₂₀H₃₇N₃O₁₃
• CAS Number: 31282-04-9
• Molecular Weight: 527.52
• MDL number: MFCD06795479
• UNSPSC 코드: 51102829

속성

• Quality Level: 100
• 분석: ≥90% (HPLC and TLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: light brown
• solubility: distilled water: ≥100 mg/mL
• 배송 상태: ambient
• 저장 온도: 2-8°C
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
• InChI key: GRRNUXAQVGOGFE-KPBUCVLVSA-N

설명

일반 설명

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).



Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).



The analytical data listed below will vary from lot to lot.



Appearance: Off-white to light brown solid

Bioassay: 1000 U/mg

Solubility: ≥100 mg/ml in H₂O

Purity (HPLC): ≥90%

Cytotoxicity: Tested in HeLa and/or CHO cells

Potency: ≥1000 µ/mg solid.

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.

경고

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

기타 정보

Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Bilang, R., et al. 1991. Gene100, 247.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330
• 예방조치 성명서: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable