324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• 동의어(들): Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• CAS Number: 72561-05-8
• 효소 위원회 번호: 3.4.21.50 (BRENDA, IUBMB)
• MDL number: MFCD02253020
• UNSPSC 코드: 12352202

속성

• Grade: Proteomics Grade
• Quality Level: 100
• 분석: ≥90% (SDS-PAGE)
• 형태: lyophilized
• 특이 활성도: ≥200 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic)
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

경고

Toxicity: Harmful (C)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

물리적 형태

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

재구성

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

법적 정보

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