264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

• 동의어(들): Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
• 실험식(Hill 표기법): C₃₆H₃₈F₃N₅O₁₄
• CAS Number: 1177935-21-5
• Molecular Weight: 821.71
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: white to off-white
• solubility: DMSO: 10 mg/mL
• 형광: λ_ex ~400 nm; λ_em ~505 nm
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

경고

Toxicity: Standard Handling (A)

서열

Z-Asp-Glu-Val-Asp-AFC

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

기타 정보

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable