251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• 동의어(들): DAR-4M AM, Diaminorhodamine-4M AM
• 실험식(Hill 표기법): C₂₈H₃₁IN₄O₅
• Molecular Weight: 630.47
• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 형태: solution
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: dark red
• 형광: λ_ex ~560 nm; λ_em ~575 nm
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

생화학적/생리학적 작용

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

포장

Packaged under inert gas

경고

Toxicity: Irritant (B)

물리적 형태

In DMSO.

재구성

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

기타 정보

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

법적 정보

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F
• Flash Point (°C): 87 °C