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Merck
모든 사진(1)

주요 문서

227036

Sigma-Aldrich

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

동의어(들):

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.51

product name

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.

Quality Level

분석

≥95% (SDS-PAGE)

형태

solid

제조업체/상표

Calbiochem®

저장 조건

do not freeze

배송 상태

ambient

저장 온도

2-8°C

일반 설명

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.
Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
Useful for tissue culture applications.

생화학적/생리학적 작용

Cell permeable: no
Primary Target
α-subunit of G-proteins
Product does not compete with ATP.
Reversible: no

포장

Please refer to vial label for lot-specific concentration.
yes

경고

Toxicity: Highly Toxic (H)

물리적 형태

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

재구성

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

분석 메모

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
single band by disc gel electrophoresis

기타 정보

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

법적 정보

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

Skull and crossbones

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3


시험 성적서(COA)

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