16-663

Magna ChIP^® Protein A+G Magnetic Beads

provides a rapid, reproducible and efficient collection of immunocomplexes for ChIP and RIP assays

• 동의어(들): ChIP magnetic A+G beads, ChIP magnetic A/G beads, ChIP magnetic beads
• UNSPSC 코드: 41105501
• NACRES: NA.84

속성

• Quality Level: 100
• 포장: pkg of 1 mL
• 제조업체/상표: Magna ChIP^®
• 저장 조건: do not freeze
• 입자 크기: ~3 μm
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.

애플리케이션

This blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays.

Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.

Used to detect/quantify: Protein A+G

품질

Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP^® A Kit (Cat. #17-610).

물리적 형태

Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween^®-20 and 0.09% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.

법적 정보

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°C): Not applicable