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Merck
모든 사진(2)

주요 문서

16-454

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

clone PY20, Upstate®, from mouse

동의어(들):

PY20, anti-phosphotyrosine antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

peroxidase conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

PY20, monoclonal

종 반응성(상동성에 의해 예측)

all

제조업체/상표

Upstate®

기술

ELISA: suitable
western blot: suitable

동형

IgG2b

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... PID1(55022)

일반 설명

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

특이성

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

면역원

Epitope: phosphotyrosine
Phosphotyrosine conjugated to carrier protein

애플리케이션

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

품질

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

표적 설명

Varies

물리적 형태

100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Protein A purified

저장 및 안정성

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

법적 정보

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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픽토그램

Exclamation markEnvironment

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Becky K Brisson et al.
PloS one, 7(9), e45588-e45588 (2012-10-03)
Insulin-like growth factor-I (IGF-I) is an essential growth factor that regulates the processes necessary for cell proliferation, differentiation, and survival. The Igf1 gene encodes mature IGF-I and a carboxy-terminal extension called the E-peptide. In rodents, alternative splicing and post-translational processing

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