14-865

p53-GST (recombinant protein)

• 동의어(들): transformation related protein 53
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

속성

• 생물학적 소스: human
• Quality Level: 100
• 분석: ~95% (SDS-PAGE)
• 양식: liquid
• 유통기한: 1 yr
• 분자량: M_w 72 kDa
• 제조업체/상표: Upstate^®
• 농도: 0.5 mg/mL
• 기술: activity assay: suitable
• solubility: water: soluble
• NCBI 수납 번호: NP_000537
• UniProt 수납 번호: P04637
• 배송 상태: dry ice
• 유전자 정보: human ... TP53(120407068)

설명

일반 설명

Research area: Apoptosis

N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography. Product Source: Full-length human p53 protein, expressed in e. coli. p53 protein is encoded by the tumor suppressor gene TP53 gene mapped on chromosome 17.

생화학적/생리학적 작용

In the cell, the p53 protein binds to DNA, leading to the activation of another gene that produces the p21 protein. This protein interacts with a cell division-stimulating protein (cdk2), preventing the cell from progressing to the next stage of cell division. Mutation in p53 protein leads to uncontrolled cell division resulting in tumor formation. The p53 protein is suggested to impede the phenotypic and genomic changes linked to cancer development through its intricate interaction with various signaling pathways including cell division, genomic stability maintenance, apoptosis, autophagy, immune response, and tumor microenvironment regulation.

품질

Routinely evaluated by SDS-PAGE and Coomassie Stain

물리적 형태

Glutathione agarose chromatography

Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.

저장 및 안정성

1 year at -20°C

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2