14-452-D

PDK1

• UNSPSC 코드: 12352200
• eCl@ss: 32160405
• NACRES: NA.27

속성

• 설명: protein target PDK1
• Quality Level: 300
• 분석: ≥80%
• 특이 활성도: 5.02 pmol/min-μg
• 농도: 0.5 mg/mL
• 기술: activity assay: suitable
• UniProt 수납 번호: O15530
• 유전자 정보: human ... PDPK1(5170)

설명

일반 설명

FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
SIZE: 556 amino acids; 63152 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.

품질

Evaluated by Kinase assay: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 EM ATP, 0.01% Brij-35 and 2 EM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.

물리적 특성

89 kDa

물리적 형태

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

저장 및 안정성

Stable for > 6 month at –80°C from date of receipt.

면책조항

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