14-448-M

Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in baculovirus infected Sf21 cells
• 제품 라인: Upstate^®
• 특이 활성도: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 분자량: M_w 35 kDa
• 정제법: (The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose)
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• 수납 번호(accession number): (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. ); NM_001798.2
• NCBI 수납 번호: NM_001798.2; NM_052827.1
• UniProt 수납 번호: P24941

설명

일반 설명

C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A

생화학적/생리학적 작용

Protein Target: CDK2/cyclinA

Target Sub-Family: CMGC

포장

Also available in 10 μg size and in bulk

Also available in 250μg size, please inquire for pricing and availability.

품질

routinely evaluated by phosphorylation of Histone H1

표적 설명

CDK2/cyclinA

결합

Replaces: 14-407

물리적 형태

The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose^™ and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose

저장 및 안정성

6 months at -70°C

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°C): Not applicable