1.25055

Fractogel^® EMD TMAE Hicap (M)

MiniChrom Column Fractogel^® TMAE Hicap (M), volume 1 mL, prepacked column

• 동의어(들): MiniChrom Column Fractogel^® TMAE Hicap (M), 1 ml
• UNSPSC 코드: 41115711

속성

• ligand: (Trimethylammoniumethyl)
• material: polypropylene housing
• 제품 라인: Fractogel^®
• 형태: prepacked column
• 파라미터: ≤8 bar pressure; ~180 cm/hr max. flow rate
• column I.D. × L: 8 mm × 20 mm
• 용적: 1 mL
• 기질 활성군: Methacrylate
• 평균 입자 크기: 40-90 μm
• 용량: ~180 mg, BSA binding capacity
• 응용 분야: mAb; recombinant protein; vaccine development
• 분리 기술: anion exchange
• 저장 온도: 15-25°C

설명

일반 설명

8 x 20 mm

특징 및 장점

MiniChrom Column Fractogel^® TMAE Hicap (M), 1 ml enables users to:

• Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior of Fractogel^® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

분석 메모

Appearance: passes test

법적 정보

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10-13 - German Storage Class 10 to 13