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Merck
모든 사진(1)

주요 문서

113200

Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 생산 유형

primary antibodies

클론

1A4, monoclonal

형태

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

baboon, rabbit, human

제조업체/상표

Calbiochem®

저장 조건

do not freeze

동형

IgG2a

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... ACTA1(58)

일반 설명

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
  • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    면역원

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    애플리케이션

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    포장

    Please refer to vial label for lot-specific concentration.

    경고

    Toxicity: Standard Handling (A)

    물리적 형태

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    분석 메모

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    기타 정보

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    법적 정보

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 2

    Flash Point (°F)

    Not applicable

    Flash Point (°C)

    Not applicable


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    문서 라이브러리 방문

    Aya Nomura-Kitabayashi et al.
    Methods in molecular biology (Clifton, N.J.), 1816, 253-268 (2018-07-11)
    We introduced the vascular remodeling mouse system induced by the wire injury to investigate the molecular and cellular mechanisms of cardiovascular diseases. Using these models, we focus on the adventitial cell population in the outermost layer of the adult vasculature
    Tobias Reicherzer et al.
    American journal of physiology. Lung cellular and molecular physiology, 315(1), L87-L101 (2018-04-13)
    Mesenchymal stromal cells (MSCs) are released into the airways of preterm infants following lung injury. These cells display a proinflammatory phenotype and are associated with development of severe bronchopulmonary dysplasia (BPD). We aimed to characterize the functional properties of MSCs
    Xiaoliang Yan et al.
    Experimental and therapeutic medicine, 23(4), 292-292 (2022-03-29)
    The recently identified adipocytokine omentin was previously found to be expressed mainly in human omental and visceral adipose tissues. As such, reduced plasma concentrations of omentin were revealed to be associated with increased risks of cardiovascular diseases. Omentin has also
    Bridget M Seitz et al.
    American journal of physiology. Heart and circulatory physiology, 313(3), H676-H686 (2017-06-20)
    Serotonin [5-hydroxytryptamine (5-HT)] causes relaxation of the isolated superior mesenteric vein, a splanchnic blood vessel, through activation of the 5-HT7 receptor. As part of studies designed to identify the mechanism(s) through which chronic (≥24 h) infusion of 5-HT lowers blood
    Juan Tang et al.
    Science signaling, 14(706), eabe3410-eabe3410 (2021-10-27)
    In response to microbes and other danger signals, the NLRP3 inflammasome in immune cells triggers the activation of the protease caspase-1, which mediates the maturation of the inflammatory cytokine IL-1β. Here, we investigated how the NLRP3 inflammasome is regulated. We

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