1.09293

KOVACS′ indole reagent

for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology

• 동의어(들): 4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles
• UNSPSC 코드: 41106212
• NACRES: NA.21

속성

• Quality Level: 200
• 양식: liquid
• 포장: pkg of 100 mL
• 기술: microbe id | specific enzyme detection: suitable
• 색상: yellow
• pH: <1 (20 °C in H₂O)
• 전이 온도: flash point 36 °C
• density: 0.92 g/cm³ at 20 °C
• 응용 분야: microbiology
• 저장 온도: 2-8°C
• 적합성: Escherichia coli; enterovirulent E. coli (EEC) spp.

설명

일반 설명

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

애플리케이션

Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

저장 및 안정성

No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition

분석 메모

Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).

기타 정보

The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 예방조치 성명서: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point (°F): 96.8 °F
• Flash Point (°C): 36 °C