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Key Documents

07-748-AF488

Sigma-Aldrich

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor™ 488 conjugate

from rabbit, ALEXA FLUOR™ 488

동의어(들):

H4K20me1, Histone H4, Lys20 monomethylated, H4 histone family member, Lys20 monomethylated

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모든 사진(2)

About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

100

결합

ALEXA FLUOR™ 488

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human

기술

immunocytochemistry: suitable

NCBI 수납 번호

NM_001034077.4

UniProt 수납 번호

P62805

타겟 번역 후 변형

monomethylation (Lys20 )

유전자 정보

human ... HIST2H4B(554313)

일반 설명

Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.

특이성

Broad species cross-reactivity expected due to sequence homology.

애플리케이션

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor™ 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H4 Lys20 monomethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in A431 cells.

표적 설명

~10 kDa observed.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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