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Merck
모든 사진(1)

Key Documents

AP180P

Sigma-Aldrich

Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed

Chemicon®, from donkey

동의어(들):

Anti-Rabbit IgG HRP, HRP Donkey Anti-Rabbit IgG

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.46

생물학적 소스

donkey

Quality Level

결합

peroxidase conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

secondary antibodies

클론

polyclonal

종 반응성

goat

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

애플리케이션

Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

품질

Routinely tested on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

물리적 형태

Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.

저장 및 안정성

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3


시험 성적서(COA)

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