07-500

Anti-p47-phox (mouse) Antibody

Upstate^®, from rabbit

• 동의어(들): Cyclin-dependent kinase inhibitor p27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: mouse
• 제조업체/상표: Upstate^®
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_000256
• UniProt 수납 번호: Q09014
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CDKN1B(1027)

설명

일반 설명

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

특이성

Recognizes mouse p47-phox, MW ~47 kDa.

면역원

KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.

애플리케이션

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse).

품질

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.

표적 설명

~47 kDa

결합

Replaces: 04-240

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.

분석 메모

Control
RAW 264.7 cell lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1