추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, chicken
종 반응성(상동성에 의해 예측)
yeast (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
제조업체/상표
Upstate®
기술
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
Saccharomyces cerevisiae ... Htb2(852284)
human ... H2BC1(255626)
일반 설명
Histone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
특이성
This rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.
면역원
KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.
애플리케이션
Anti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.
품질
Evaluated by Western Blotting in Chicken Core Histone.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
표적 설명
~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
결합
Replaces: MABE15
물리적 형태
Format: Purified
Protein A chromatography
Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.
저장 및 안정성
Stable for 1 year at -20ºC from date of receipt.
분석 메모
Control
Acid extract from either sodium butyrate or colcemid treated HeLa cells.
Acid extract from either sodium butyrate or colcemid treated HeLa cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Histone variant macroH2A1 deletion in mice causes female-specific steatosis.
Boulard, M; Storck, S; Cong, R; Pinto, R; Delage, H; Bouvet, P
Epigenetics & Chromatin null
Marco De Cecco et al.
Aging, 3(10), 955-967 (2011-10-19)
Replicative cellular senescence was discovered some 50 years ago. The phenotypes of senescent cells have been investigated extensively in cell culture, and found to affect essentially all aspects of cellular physiology. The relevance of cellular senescence in the context of
dRYBP counteracts chromatin-dependent activation and repression of transcription.
Fereres, S; Simon, R; Mohd-Sarip, A; Verrijzer, CP; Busturia, A
Testing null
Wei-Ting Liao et al.
Carcinogenesis, 30(6), 1064-1072 (2009-04-21)
Bowen's disease (BD), a carcinoma in situ of the skin, has been identified as an early lesion in arsenic carcinogenesis. Patients with arsenic-induced Bowen's disease (As-BD) showed both cutaneous and systemic immune dysfunctions. We set out to evaluate the interactions
Miia M Rytinki et al.
Cellular and molecular life sciences : CMLS, 68(19), 3219-3232 (2011-01-22)
Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal
문서
Loading controls in western blotting application.
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