07-329

Anti-acetyl-Histone H4 (Lys16) Antibody

Upstate^®, from rabbit

• 동의어(들): Histone H4 Acetylated on Lysine 16, H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, rat, human
• 제조업체/상표: Upstate^®
• 기술: ChIP: suitable (ChIP-seq); dot blot: suitable; inhibition assay: suitable (peptide); multiplexing: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_175054
• UniProt 수납 번호: P62805
• 배송 상태: wet ice
• 타겟 번역 후 변형: acetylation (Lys16)
• 유전자 정보: human ... H4C1(8359) ; mouse ... H4C1(326619)

설명

일반 설명

Histone H4, Acetylated on Lysine 16 (UniProt: P62805; also known as H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a) is encoded by the HIST1H4A (also known as H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, HIST1H4I, H4/N, HIST1H4B) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 features a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. Chromosomal aberrations involving histone H4 is a cause of B-cell non-Hodgkin lymphomas.

특이성

Broad species cross-reactivity is expected.

This rabbit polyclonal antibody detects human Histone H4 acetylated on Lysine 16.

면역원

Epitope: N-terminus (Lys 16)

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4 acetylated on Lysine 16.

애플리케이션

Anti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq.

Chromatin Immunoprecipitation Analysis: 1 ug from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in HeLa chromatin.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in

an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

품질

Evaluated by Western Blotting in lysate from Sodium Butyrate treated HeLa cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells.

표적 설명

10 kDa

물리적 형태

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8° C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable