07-225

Anti-TrkB Antibody

Upstate^®, from rabbit

• 동의어(들): BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, neurotrophic tyrosine kinase receptor, type 2, tyrosine kinase receptor B
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: WB
• 종 반응성: mouse, rat
• 기술: western blot: suitable
• citations: 83

속성

• 생물학적 소스: rabbit
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: mouse, rat
• 제조업체/상표: Upstate^®
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_036863
• UniProt 수납 번호: Q16620
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NTRK2(4915)

설명

일반 설명

The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.

특이성

Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.

면역원

The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.

애플리케이션

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

This Anti-TrkB Antibody is validated for use in WB for the detection of TrkB.

품질

Evaluated by western blot on mouse brain membrane protein preparations.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.

표적 설명

145 κDa

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.

분석 메모

Control
Brain tissue.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1