07-2113

Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)

from rabbit, purified by affinity chromatography

• 동의어(들): 40S ribosomal protein S6, Phosphoprotein NP33
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_001001
• UniProt 수납 번호: P62753
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer240/pSer244)
• 유전자 정보: human ... RPS6(6194)

설명

일반 설명

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.

특이성

This antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

면역원

Epitope: Phosphorylated Ser240/Ser244

KLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

애플리케이션

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

This phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.

품질

Evaluated by Western Blot in PMA treated and untreated A431 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.

표적 설명

~32 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
PMA treated and untreated A431 cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable