추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
pig, human
종 반응성(상동성에 의해 예측)
porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), monkey (based on 100% sequence homology), opossum (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
기술
immunocytochemistry: suitable
western blot: suitable
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ECT2(1894)
일반 설명
Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC.
특이성
This antibody recognizes ECT2 at the
C-terminus.
C-terminus.
면역원
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus.
애플리케이션
Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC.
Research Category
Signaling
Signaling
Research Sub Category
G-proteins
G-proteins
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected ECT2 in A549 cell lysate.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu)
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu)
품질
Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate.
표적 설명
~ 105 kDa observed
물리적 형태
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
A549 cell lysate
A549 cell lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Danielle R Cook et al.
Genes & cancer, 2(10), 932-942 (2012-06-16)
Ect2 is a member of the human Dbl family of guanine nucleotide exchange factors (RhoGEFs) that serve as activators of Rho family small GTPases. Although Ect2 is one of at least 25 RhoGEFs that can activate the RhoA small GTPase
Ming Yi et al.
Experimental hematology & oncology, 11(1), 107-107 (2022-12-27)
Breast cancer is the most common malignancy and the second leading cause of cancer-related death in women. Recent studies have indicated that aberrant activation of Rho GTPases relates to the malignant properties of breast cancer cells. As the guanine nucleotide
Laurent Guillemot et al.
Molecular biology of the cell, 25(13), 1995-2005 (2014-05-09)
The regulation of Rho-family GTPases is crucial to direct the formation of cell-cell junctions and tissue barriers. Cingulin (CGN) and paracingulin (CGNL1) control RhoA activation in epithelial cells by interacting with RhoA guanidine exchange factors. CGNL1 depletion also inhibits Rac1
Bin Liu et al.
Translational oncology, 11(3), 700-714 (2018-04-10)
Pancreatic cancer is the fourth leading cause for cancer-related death, and early diagnosis is one key to improve the survival rate of this disease. Molecular biomarkers are an important method for diagnostic use in pancreatic cancer. We used data from
Yi Liu et al.
Cell reports, 30(3), 771-782 (2020-01-23)
Lung squamous cell carcinoma (LSCC) is a prevalent form of lung cancer exhibiting distinctive histological and genetic characteristics. Chromosome 3q26 copy number gain (CNG) is a genetic hallmark of LSCC present in >90% of tumors. We report that 3q26 CNGs
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