추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rat, human, mouse
제조업체/상표
Upstate®
기술
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... PRKCE(5581)
일반 설명
Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.
특이성
Predicted to cross-react with rabbit.
Recognizes PKCε, Mr 95 kDa.
면역원
KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.
애플리케이션
Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.
Immunoprecipitation:
An independent lab has reported this antibody immunoprecipitates PKCε.
Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
An independent lab has reported this antibody immunoprecipitates PKCε.
Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
품질
Routinely evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.
표적 설명
Approx. 95
물리적 형태
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Store at -20°C.
Store at -20°C.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Two conventional protein kinase C isoforms, alpha and beta I, are involved in the ATP-induced activation of volume-regulated anion channel and glutamate release in cultured astrocytes.
Alena Rudkouskaya,Artur Chernoguz,Renee E Haskew-Layton,Alexander A Mongin
Journal of Neurochemistry null
Post-ischaemic activation of kinases in the pre-conditioning-like cardioprotective effect of the platelet-activating factor.
Penna C, Mognetti B, Tullio F, Gattullo D, Mancardi D, Moro F, Pagliaro P, Alloatti G
Acta Physiologica (Oxford, England) null
Protein kinase C epsilon activates lens mitochondrial cytochrome c oxidase subunit IV during hypoxia.
Michael Barnett, Dingbo Lin, Vladimir Akoyev, Lloyd Willard, Dolores Takemoto et al.
Experimental Eye Research null
Platelet-activating factor induces cardioprotection in isolated rat heart akin to ischemic preconditioning: role of phosphoinositide 3-kinase and protein kinase C activation.
Penna, C; Alloatti, G; Cappello, S; Gattullo, D; Berta, G; Mognetti, B; Losano, G; Pagliaro, P
American Journal of Physiology. Heart and Circulatory Physiology null
Neuroprotective preconditioning of rat brain cultures with ethanol: potential transduction by PKC isoforms and focal adhesion kinase upstream of increases in effector heat shock proteins.
Sreevidya Sivaswamy,Edward J Neafsey,Michael A Collins
The European Journal of Neuroscience null
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