05-716-I

Anti-Myosin Heavy Chain Antibody, clone A4.1025

clone A4.1025

• 동의어(들): MyHC-2x, MyHC-IIx/d, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin heavy chain skeletal muscle adult 1, Myosin-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• Quality Level: 200
• 생물학적 소스: mouse
• 항체 형태: purified antibody
• 클론: A4.1025, monoclonal
• 분자량: ~220 kDa
• 정제법: using protein G
• 종 반응성: mouse, human, rat
• 동형: IgG2aκ
• 수납 번호(accession number): NP_005954
• UniProt 수납 번호: P12882

설명

일반 설명

Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). The globular head of myosin heavy chain contains the actin-activated ATPase. It has been reported that during rest, the myosin head does interact with the actin filament due to the presence of tropomyosin. However, when muscle contracts, tropomyosin shifts out of the way to allow the globular head to engage the actin filaments. Myosin heavy chain is a conventional myosin with a N-terminal SH3-like domain (aa 37-82), a myosin motor domain (aa 86-782), and an actin-binding domain (aa 761-775). It has a rod like tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils

특이성

Clone A4.1025 is a mouse monoclonal antibody that detects Myosin Heavy Chain.

면역원

Partially purified myosin from adult human skeletal muscle.

애플리케이션

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin Heavy Chain in lysates from L6 cells and human skeletal muscle.

mmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.

Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications

Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications

Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications

Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

품질

Evaluated by Western Blotting in C2C12 cell lysate.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Stable for 1 year at +2°C to +8°C from date of receipt.

기타 정보

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable