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![E-64 N-[N-(L-3-Trans-carboxirane-2-carbonyl)-L-leucyl]-agmatine](/deepweb/assets/sigmaaldrich/product/structures/168/240/c77e3f6a-6709-4d17-b774-b850e8a54a51/640/c77e3f6a-6709-4d17-b774-b850e8a54a51.png)
특이성
Recognizes α-actinin.
면역원
Human α-actinin
애플리케이션
Immunoprecipitation is not recommended
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.
품질
Routinely evaluated in immunoblot on human A431 cells
표적 설명
100 kDa
물리적 형태
Format: Purified
Please see product datasheet for lot-specific formulation.
Protein A purified
저장 및 안정성
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy.
Bian, Z; Liao, H; Zhang, Y; Wu, Q; Zhou, H; Yang, Z; Fu, J; Wang, T; Yan, L; Shen, D; Li, H; Tang, Q
Testing null
IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis.
Dai, J; Shen, DF; Bian, ZY; Zhou, H; Gan, HW; Zong, J; Deng, W; Yuan, Y; Li, F; Wu et al.
Testing null
Jing Zong et al.
Laboratory investigation; a journal of technical methods and pathology, 93(10), 1128-1136 (2013-08-21)
Nucleotide-binding oligomerization domain-2 (NOD2, also designated CARD15), a member of the NOD-leucine-rich repeat (LRR) protein family (also called the CATERPILLAR family), is upregulated in atheroma lesions and has an important role in regulating the intracellular recognition of bacterial components by
Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function
Fukami, K, et al
Nature, 359, 150-152 (1992)
Morgane Macheret et al.
Nature, 555(7694), 112-116 (2018-02-22)
Oncogene-induced DNA replication stress contributes critically to the genomic instability that is present in cancer. However, elucidating how oncogenes deregulate DNA replication has been impeded by difficulty in mapping replication initiation sites on the human genome. Here, using a sensitive
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