추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
3F2, monoclonal
종 반응성
bovine, mouse, hamster, rat, human
제조업체/상표
Upstate®
기술
immunohistochemistry: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... GRB2(2885)
특이성
N-terminal SH3 region of GRB2
면역원
Recombinant GRB2/Ash
애플리케이션
This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.
품질
routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract
표적 설명
23-25kDa
물리적 형태
Format: Purified
PBS containing 50% glycerol
분석 메모
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
Wang, Y., et al
The Journal of Biological Chemistry, 271, 17-20 (1996)
A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
Rucci, N; DiGiacinto, C; Orru, L; Millimaggi, D; Baron, R; Teti, A
Journal of Cell Science null
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
Y Chen et al.
The Journal of biological chemistry, 273(31), 19809-19816 (1998-07-25)
While it is well established that adenylyl cyclase and phospholipase C-beta are two proximal signal effectors for the calcitonin receptor, the more distal signaling pathways are less well characterized. G protein-coupled receptors can activate Erk1/2 by Gs-, Gi-, or Gq-dependent
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