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Merck
모든 사진(4)

Key Documents

05-175

Sigma-Aldrich

Anti-Myb Antibody, clone 1-1

clone 1-1, Upstate®, from mouse

동의어(들):

Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA), v-myb avian myeloblastosis viral oncogene homolog, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral on

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

1-1, monoclonal

종 반응성

mouse, human

제조업체/상표

Upstate®

기술

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... MYB(4602)

일반 설명

Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5′-YAAC[GT]G-3′. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

특이성

This antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).

면역원

Recombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.

애플리케이션

Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.

Immunohistochemistry:
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

품질

Routinely evaluated on RIPA lysate from Jurkat cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.

표적 설명

80 kDa

물리적 형태

Ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography.
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Marc R Mansour et al.
The Journal of experimental medicine, 210(8), 1545-1557 (2013-07-17)
The oncogenic transcription factor TAL1/SCL is aberrantly expressed in 60% of cases of human T cell acute lymphoblastic leukemia (T-ALL) and initiates T-ALL in mouse models. By performing global microRNA (miRNA) expression profiling after depletion of TAL1, together with genome-wide
Zhenglong Wu et al.
Journal of experimental & clinical cancer research : CR, 32, 98-98 (2013-12-03)
Mir-29 microRNA families are involved in regulation of various types of cancers. Although Mir-29 was shown to play an inhibitory role in tumorigenesis, the role of Mir-29 in breast cancer still remains obscure. In this study, we showed that Mir-29a
In vitro and in vivo reversal of multidrug resistance in a human leukemia-resistant cell line by mdr1 antisense oligodeoxynucleotides.
C Cucco, B Calabretta
Cancer Research null
Delivery of c-myb antisense oligodeoxynucleotides to human neuroblastoma cells via disialoganglioside GD(2)-targeted immunoliposomes: antitumor effects.
G Pagnan, D D Stuart, F Pastorino, L Raffaghello, P G Montaldo, T M Allen, B Calabretta, M Ponzoni
Journal of the National Cancer Institute null
Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity
Salomoni, P., et al
Proceedings of the National Academy of Sciences of the USA, 94, 3296-3301 (1997)

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