콘텐츠로 건너뛰기
Merck
모든 사진(2)

Key Documents

추가 자료

M0696

Sigma-Aldrich

Anti-MNK2 (N-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-GPRK7, Anti-MAP kinase interacting serine/threonine kinase 2, Anti-MKNK2

로그인조직 및 계약 가격 보기
모든 사진(2)

About This Item

UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen ~50 kDa

종 반응성

rat (predicted), human, mouse (predicted)

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

농도

~1.0 mg/mL

기술

immunocytochemistry: 2-5 μg/mL using paraformadehyde fixed HEK-293T cells transfected with human MNK2
immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells transfected with human MNK2
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human MNK2

UniProt 수납 번호

Q9HBH9

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... MKNK2(2872)
mouse ... Mknk2(17347)
rat ... Mknk2(299618)

일반 설명

MNK2 is a serine threonine kinase that phosphorylates endogenous eIF4E and thereby regulates protein translation and cell growth. This kinase inhibits apoptotic responses induced by arsenic trioxide. MNK2 has two distinct splice forms, namely; MNK2a and MNK2b. The localization and function of MNK2 splice variants are directed by their N and C termini . Anti-MNK2 (N-terminal) antibody is specific for human MNK2. The antibody is also expected to react with mouse and rat MNK2. In immunoblotting, staining of the MNK2 band is specifically inhibited by the immunizing peptide.

면역원

synthetic peptide corresponding to amino acids 51-64 of human MNK2 (corresponding to isoforms 1 and 2), conjugated to KLH via a C-terminal added cysteine residue. The corresponding sequence is conserved in human, rat, and mouse.

애플리케이션

Anti-MNK2 (N-terminal) antibody is suitable for use in immunocytochemistry (2-5 μg/mL using paraformaldehyde fixed, MNK2-transfected, HEK-293T cells) and indirect immunofluorescence. The antibody may also be used for immunoprecipitation (2-4 μg) and western blot (1-2 μg/mL) using lysates of HEK-293T cells transfected with human MNK2.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Y Shi et al.
Oncogene, 32(2), 190-197 (2012-03-01)
When mTOR inhibitor rapalogs prevent cap-dependent translation of cell-cycle proteins like c-myc, continuing tumor cell growth depends on cap-independent translation, which is mediated by internal ribosome entry sites (IRESes) located in the 5'-UTR (untranslated region) of transcripts. To investigate if
Senthilmurugan Ramalingam et al.
Cancers, 11(3) (2019-03-06)
Currently, there are no effective therapies for patients with triple-negative breast cancer (TNBC), an aggressive and highly metastatic disease. Activation of eukaryotic initiation factor 4E (eIF4E) by mitogen-activated protein kinase (MAPK)-interacting kinases 1 and 2 (Mnk1/2) play a critical role
Gert C Scheper et al.
Molecular and cellular biology, 23(16), 5692-5705 (2003-08-05)
The cap-binding eukaryotic initiation factor eIF4E is phosphorylated by the mitogen-activated protein (MAP) kinase-interacting kinases (Mnk's). Three forms of the Mnk's exist in human cells: Mnk1, Mnk2a, and Mnk2b. These last two are derived from the same gene by alternative
G C Scheper et al.
Molecular and cellular biology, 21(3), 743-754 (2001-01-12)
The cap-binding translation initiation factor eukaryotic initiation factor 4E (eIF4E) is phosphorylated in vivo at Ser209 in response to a variety of stimuli. In this paper, we show that the mitogen-activated protein kinase (MAPK) signal-integrating kinase Mnk2 phosphorylates eIF4E at
U Knauf et al.
Molecular and cellular biology, 21(16), 5500-5511 (2001-07-21)
Eukaryotic initiation factor 4E (eIF4E) is a key component of the translational machinery and an important modulator of cell growth and proliferation. The activity of eIF4E is thought to be regulated by interaction with inhibitory binding proteins (4E-BPs) and phosphorylation
모든 관련된 서류 보기

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.