05-1424

Anti-Endoglin Antibody, clone P3D1

clone P3D1, from mouse

• 동의어(들): CD105, Ancillary TGF-beta Receptor
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P3D1, monoclonal
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): rat
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2a
• NCBI 수납 번호: NM_000118.1
• UniProt 수납 번호: P17813
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ENG(2022) ; mouse ... Eng(13805) ; rat ... Eng(497010)

설명

일반 설명

Endoglin is a homodimeric integral membrane glycoprotein comprised of 95 kDa subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family ligands to signaling receptors. Endoglin binds TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. It is also expressed by U937 cells and activated macrophages. Endoglin expression is up regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

특이성

Reacts with human endoglin.

면역원

Human resting umbilical vein endothelial cells.

애플리케이션

Anti-Endoglin Antibody, clone P3D1 is an antibody against Endoglin for use in WB, IH, IC, IP, FC, ELISA.

Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue.

ELISA: A previous lot of this antibody was used in ELISA.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunocytochemistry: A previous lot of this antibody was used in IC.

Flow Cytometry: A previous lot of this antibody was used in FC.

Optimal working dilutions must be determined by the end user.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

품질

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ENDOGLIN on 10 μg of Jurkat lysates.

표적 설명

~71 kDa

결합

Replaces: MAB2152

물리적 형태

Format: Purified

Purified

Purified mouse monoclonal IgG2a in 0.02M phosphate buffer, 0.25M sodium chloride, 0.1% sodium azide, pH 7.6

저장 및 안정성

Stable at 2-8°C for up to 1 year from date of receipt.

분석 메모

Control
Jurkat cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable