850306C

07:0 PC (DHPC)

1,2-diheptanoyl-sn-glycero-3-phosphocholine, chloroform

• 동의어(들): DHPC; PC(7:0/7:0)
• 실험식(Hill 표기법): C₂₂H₄₄NO₈P
• CAS Number: 39036-04-9
• Molecular Weight: 481.56
• UNSPSC 코드: 51191904
• NACRES: NA.25

속성

• 분석: >99% (TLC)
• 양식: liquid
• 포장: pkg of 1 × 20 mL (850306C-500mg); pkg of 2 × 4 mL (850306C-200mg)
• 제조업체/상표: Avanti Research^™ - A Croda Brand 850306C
• 농도: 25 mg/mL (850306C-200mg); 25 mg/mL (850306C-500mg)
• 배송 상태: dry ice
• 저장 온도: −20°C
• SMILES string: [P](=O)([O-])(OC[C@H](OC(=O)CCCCCC)COC(=O)CCCCCC)OCC[N+](C)(C)C
• InChI: 1S/C22H44NO8P/c1-6-8-10-12-14-21(24)28-18-20(31-22(25)15-13-11-9-7-2)19-30-32(26,27)29-17-16-23(3,4)5/h20H,6-19H2,1-5H3/t20-/m1/s1
• InChI key: RBFSPQDASPEAID-HXUWFJFHSA-N

설명

일반 설명

07:0 PC (DHPC)/1,2-diheptanoyl-sn-glycero-3-phosphocholine, is a phospholipidwhich functions as a detergent. Phosphatidylcholine (PC) is a strong bilayer-forming lipid. It the most common phospholipid in mammalian membranes. It is also an important component of the mucosal layer of the colon.

The list of Phosphatidylcholine products offered by Avanti is designed to provide compounds having a variety of physical properties. Products available include short chain (C3-C8 are water soluble and hygroscopic), saturated, multi-unsaturated and mixed acid PC′s. All of the products are purified by HPLC, and special precautions are taken to protect the products for oxidization and hydrolysis. Preserve the Activity of Solubilized Membrane ProteinsProtein Preservation DHPC preserves the native conformation and therefore the activity of the solubilized proteins. The three dimensional structure and activity of proteins are retained not only at the 10-15mM DHPC concentrations at which maximum solubilization usually occurs, but also over a large range of DHPC concentrations (up to 40mM). Not only is the three dimensional structure and hence the activity of most protein retained in excess DHPC, but the proteins also appear to be stable in DHPC. Such a result suggests that the direct interaction of DHPC with integral membrane proteins is weak. If the interaction of DHPC with membrane proteins is indeed weak, it follows that intrinsic membrane lipids will remain associated with the membrane proteins. Solubilization Mechanism DHPC is thought to exert a wedge-like effect on the neighboring lipids, mainly due to its bulky polar group and short hydrocarbon chains. This produces membrane destabilization at relatively low DHPC concentrations. All membranes investigated are solubilized at an identical DHPC / lipid ratio. This finding is taken as evidence that DHPC primarily interacts with the lipid bilayer and not with the membrane proteins. The principle underlying the preservation of the native protein structure is the inability of DHPC to displace intrinsic membrane lipids from integral membrane proteins.Advantages of DHPC: DHPC preserves the activity of solubilized membrane proteins. DHPC is not readily oxidized, and is stable over a wide pH range (4-10). DHPC forms micelles rather than bilayers when dispersed in water. (CMC = 1.4mM). DHPC shows a broad size distribution depending on the NaCl concentration of suspension. DHPC does not interfere with spectrophotometric measurements. DHPC is an ultra pure compound.

애플리케이션

07:0 PC (DHPC) is suitable for use:

• in the preparation of vesicles
• to test the stability of purified cytochrome b₅₅₈
• in the formation of lipid bicelles
• as an additive added to the supernatant

포장

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (850306C-500mg)

5 mL Clear Glass Sealed Ampule (850306C-200mg)

법적 정보

Avanti Research is a trademark of Avanti Polar Lipids, LLC

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• 예방조치 성명서: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• 표적 기관: Central nervous system, Liver,Kidney
• WGK: WGK 3