302643

Trypan Blue

Dye content 60 %, ≥80% (HPLC)

• 동의어(들): Direct blue 14
• Linear Formula: C₃₄H₂₄N₆O₁₄S₄Na₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• 색상 지수 번호: 23850
• Beilstein: 4360496
• EC Number: 200-786-7
• MDL number: MFCD00003969
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24858268
• NACRES: NA.47

속성

• Quality Level: 200
• 분석: ≥80% (HPLC)
• 형태: powder
• 구성: Dye content, 60%
• 기술: microbe id | staining: suitable
• 색상: dark green to brown, or Brown-Black
• mp: >300 °C (lit.)
• solubility: H₂O: 1 mg/mL
• λmax: 607 nm
• ε (흡광계수): ≥60000 at 603-607 nm in methanol at 0.01 g/L; 60000 at 603-607 nm in methanol
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J
• 유전자 정보: human ... PTPN1(5770) , PTPRF(5792)

설명

일반 설명

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

애플리케이션

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

생화학적/생리학적 작용

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:

• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
• to stain plant tissue in order to visualize cell death.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H350
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges